Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using PRAS40 antibody at 1:500 dilution.| Product Name | PRAS40 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRAS40 (AKT1 substrate 1 (proline-rich))AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRAS40. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chimpanzee, BovineRat (87%), Chimpanzee (99%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Lobe antibody MGC2865 antibody PRAS40 antibody AKT1S1 antibody proline-rich AKT1 substrate 1 antibody 40 kDa proline-rich AKT substrate antibody "proline-rich Akt substrate 40 kDa antibody" AKT1 substrate 1 (proline-rich) antibody |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (6) |
| Cellular Localization | Cytoplasm , cytosol |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using PRAS40 antibody at 1:500 dilution.| Positive Control | 293T , HeLa , NIH-3T3 , JC , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
