Application
WB analysis of extracts from various cells using PRDM14 antibody.Application
WB analysis of lysates from PA-1 cells transfected with differentt shRNAs targeting PRDM14 using PRDM14 antibody.Application
ChIP analysis was performed with 293 lysates and either PRDM14 antibody or control RABBIT IgG. The precipitated DNA was detected by qPCR with primer sets targeting to P1, P2, P3 and P4 regions of RUNX1 gene as the schematic diagram illustrated. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Application
Immunofluorescence analysis of A549 cell using PRDM14 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of A549 cell using PRDM14 antibody.Application
Immunohistochemistry of paraffin-embedded human kidney using PRDM14 antibody at dilution of 1:100 (x400 lens).Application
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PRDM14 antibody at dilution of 1:100 (x400 lens).| Product Name | PRDM14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRDM14This gene encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer. [provided by RefSeq, Dec 2011] |
| Immunogen | Recombinant protein of human PRDM14 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:20 - 1:100. CHIP: 1:20 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PFM11 PR domain containing 14 |
|---|---|
| Target | PRDM14 |
| Molecular Weight(MW) | 64 kDa (note) |
Application
WB analysis of extracts from various cells using PRDM14 antibody.
Application
WB analysis of lysates from PA-1 cells transfected with differentt shRNAs targeting PRDM14 using PRDM14 antibody.
Application
ChIP analysis was performed with 293 lysates and either PRDM14 antibody or control RABBIT IgG. The precipitated DNA was detected by qPCR with primer sets targeting to P1, P2, P3 and P4 regions of RUNX1 gene as the schematic diagram illustrated. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application
Immunofluorescence analysis of A549 cell using PRDM14 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of A549 cell using PRDM14 antibody.
Application
Immunohistochemistry of paraffin-embedded human kidney using PRDM14 antibody at dilution of 1:100 (x400 lens).
Application
Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using PRDM14 antibody at dilution of 1:100 (x400 lens).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:20 - 1:100. CHIP: 1:20 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
