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ELISA: (direct and indirect 1:2,000-1:10,000). Immunoprecipitation: (1-5 ul per sample in a final reaction volume of 0.5-1 ml). Western Blot: (1:2,000-1:10,000 using ECL suggested blocking and dilution buffer is PBST with 0.05% Tween 20 with 5% skim milk incubate 1 hour at room temperature). Optimal conditions must be determined individually for each application.:
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