Application
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADIPOR1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of widr cells using ADIPOR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ADIPOR1 Antibody in Y79 cell line lysates (35ug/lane)WB
(A) The expression of AdipoR1, AMPKα, p-AMPKα, PGC-1α in in subcutaneous adipose of pigs was detected by western blotting.WB
(A, B) RES treatment affect the protein expression of AdipoQ pathway signal genes in muscle of pigs.WB
(C, D) The protein levels of genes in AdipoQ signaling pathway.| Product Name | ADIPOR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The adiponectin receptors, ADIPOR1 and ADIPOR2, serve as receptors for globular and full-length adiponectin and mediate increased AMPK and PPAR-alpha ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin.1) Civitarese,A.E., et.al., Diabetologia 47 (5), 816-820 (2004) |
| Immunogen | This ADIPOR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-331 amino acids from the C-terminal region of human ADIPOR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADIPOR1 |
|---|---|
| Alternative Names | Adiponectin receptor protein 1 Progestin and adipoQ receptor family member I ADIPOR1 PAQR1 TESBP1A |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western Blot at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADIPOR1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of widr cells using ADIPOR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ADIPOR1 Antibody in Y79 cell line lysates (35ug/lane)
WB
(A) The expression of AdipoR1, AMPKα, p-AMPKα, PGC-1α in in subcutaneous adipose of pigs was detected by western blotting.
WB
(A, B) RES treatment affect the protein expression of AdipoQ pathway signal genes in muscle of pigs.
WB
(C, D) The protein levels of genes in AdipoQ signaling pathway.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
