Application
PRKCSH antibody [N1C1] detects PRKCSH protein at cytoplasm on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded normal liver. PRKCSH antibody [N1C1] dilution: 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PRKCSH antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:10000Application
PRKCSH antibody [N1C1] detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRKCSH protein stained by PRKCSH antibody [N1C1] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | PRKCSH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PRKCSH (protein kinase C substrate 80K-H)This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease (PCLD). Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRKCSH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (86%), Rat (87%), Pig (90%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AGE-R2 antibody G19P1 antibody PCLD antibody PLD1 antibody PRKCSH antibody hepatocystin antibody glucosidase 2 subunit beta antibody AGE-binding receptor 2 antibody "glucosidase II beta subunit antibody" glucosidase II subunit beta antibody prot |
|---|---|
| Target | PRKCSH |
| Molecular Weight(MW) | 59 kDa (note) (5) |
| Cellular Localization | Endoplasmic reticulum |
Application
PRKCSH antibody [N1C1] detects PRKCSH protein at cytoplasm on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded normal liver. PRKCSH antibody [N1C1] dilution: 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PRKCSH antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:10000
Application
PRKCSH antibody [N1C1] detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRKCSH protein stained by PRKCSH antibody [N1C1] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
