Application
Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.Application
Immunohistochemical analysis of paraffin-embedded H. small intestine section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Western blot analysis of lysates from A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right), using ADRA1D Antibody at 1:1000 at each lane.| Product Name | ADRA1D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor, Alpha-adrenergic receptor 1a, ADRA1D, ADRA1A |
| Product description | This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.1) Bruno J.F.,et al.Biochem. Biophys. Res. Commun. 179:1485-1490(1991). |
| Immunogen | This ADRA1D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30amino acids from the N-terminal region of human ADRA1D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For IHC-P starting dilution is: 1:25 For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADRA1D |
|---|---|
| Alternative Names | Alpha-1D adrenergic receptor Alpha-1A adrenergic receptor Alpha-1D adrenoreceptor Alpha-1D adrenoceptor Alpha-adrenergic receptor 1a ADRA1D ADRA1A |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Application
Immunohistochemical analysis of paraffin-embedded H. small intestine section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term). Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Western blot analysis of lysates from A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right), using ADRA1D Antibody at 1:1000 at each lane.| Application Notes | For IHC-P starting dilution is: 1:25 For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
