Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).Application
ADRA2C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ADRA2C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The mouse studies revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons. The alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes the alpha2C subtype, which contains no introns in either its coding or untranslated sequences.1) Chen, Q.J., et al. Clin. Biochem. 43(15):1201-1204(2010) |
| Immunogen | This ADRA2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-382 amino acids from the C-terminal region of human ADRA2C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-P,WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADRA2C |
|---|---|
| Alternative Names | Alpha-2C adrenergic receptor Alpha-2 adrenergic receptor subtype C4 Alpha-2C adrenoreceptor Alpha-2C adrenoceptor Alpha-2CAR ADRA2C ADRA2L2 ADRA2RL2 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application
ADRA2C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
