Application
Immunofluorescence of AES in 293 cells with AES antibody at 20 μg/mL.Application
Immunocytochemistry of AES in 293 cells with AES antibody at 10 μg/mL.Application
Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 μg/mL.| Product Name | AES Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AES Antibody: GRG, ESP1, GRG5, TLE5, AES-1, AES-2, GRG, Amino-terminal enhancer of split, Gp130-associated protein GAM, Amino enhancer of split |
| Product description | AES Antibody: Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment. Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis. It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.1) Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57. |
| Immunogen | AES antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human AES. The immunogen is located within the first 50 amino acids of AES. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| AES antibody can be used for the detection of AES by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AES |
|---|---|
| Alternative Names | AES Antibody: GRG ESP1 GRG5 TLE5 AES-1 AES-2 GRG Amino-terminal enhancer of split Gp130-associated protein GAM Amino enhancer of split |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
Application
Immunofluorescence of AES in 293 cells with AES antibody at 20 μg/mL.
Application
Immunocytochemistry of AES in 293 cells with AES antibody at 10 μg/mL.
Application
Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | AES antibody can be used for the detection of AES by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AES antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AES Antibody is supplied in PBS containing 0.02% sodium azide. |
