Application
Immunohistochemistry of AFAP1L2 in human liver tissue with AFAP1L2 antibody at 2.5 μg/mL.Application
Western blot analysis of AFAP1L2 in mouse liver tissue lysate with AFAP1L2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of AFAP1L2 in human liver tissue with AFAP1L antibody at 20 μg/mL.| Product Name | AFAP1L2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | AFAP1L2 Antibody: AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP), containing several SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and many potential phosphorylation sites. It interacts with and is phosphorylated by c-Src tyrosine kinase. Suppression of AFAP1L2 via siRNA reduced Src activity, IL-8 production, EGF-induced phosphorylation of Akt and GSK3beta, and altered cell cycles in human lung epithelial cells suggesting that AFAP1L2 plays a role as an adaptor in the regulation of Src signal transduction and multiple cellular functions. Recent experiments have shown that AFAP1L2 is highly expressed in thyroid and is the substrate RET/PTC kinase, a thyroid-specific kinase that plays a pathogenic role in papillary thyroid cancer. Down-regulation of AFAP1L2 in these cancer cells reduced Akt activity, inhibiting cell-cycle progression and cancer cell survival in suspension, indicating that AFAP1L2 may be a valuable target in thyroid cancer therapy. At least four isoforms of AFAP1L2 are known to exist.1) Xu J, Bai X-H, Lodyga M, et al. XB130, a novel adaptor protein for signal transduction. J. Biol. Chem.2007; 282:16401-12. |
| Immunogen | AFAP1L2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AFAP1L2. The immunogen is located within the last 50 amino acids of AFAP1L2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| AFAP1L2 antibody can be used for detection of AFAP1L2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AFAP1L2 |
|---|---|
| Alternative Names | AFAP1L2 Antibody: XB130 KIAA1914 CTB-1144G6.4 XB130 Actin filament-associated protein 1-like 2 AFAP1-like protein 2 |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Entrez Gene | 84632 |
|---|---|
| Protein Accession | NP_001001936 |
Application
Immunohistochemistry of AFAP1L2 in human liver tissue with AFAP1L2 antibody at 2.5 μg/mL.
Application
Western blot analysis of AFAP1L2 in mouse liver tissue lysate with AFAP1L2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of AFAP1L2 in human liver tissue with AFAP1L antibody at 20 μg/mL.| Application Notes | AFAP1L2 antibody can be used for detection of AFAP1L2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | AFAP1L2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AFAP1L2 Antibody is supplied in PBS containing 0.02% sodium azide. |
