Application
Western blot analysis of AFP in fetal human liver tissue lysate with AFP antibody at (A) 1 μg/mL and (B) 2 μg/mL.Application
Immunofluorescence of AFP in rat liver tissue with AFP antibody at 20 μg/mL.Application
Immunohistochemistry of AFP in mouse liver tissue with AFP antibody at 2.5 μg/ml.Application
Immunohistochemistry of AFP in rat liver tissue with AFP antibody at 10 μg/mL.| Product Name | AFP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein |
| Product description | AFP Antibody: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma. However, hereditary persistance of AFP may also be found in individuals with no obvious pathology. AFP is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. AFP levels in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.1) Lafuste P, Robert B, Mondon F, et al. Alpha-fetoprotein gene expression in early and full-term human trophoblast. Placenta2002; 23:600-12. |
| Immunogen | AFP antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human AFP. The immunogen is located within amino acids 340 - 390 of AFP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AFP antibody can be used for detection of AFP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AFP |
|---|---|
| Alternative Names | AFP Antibody: FETA HPAFP Alpha-fetoprotein Alpha-1-fetoprotein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (91%) |
Application
Western blot analysis of AFP in fetal human liver tissue lysate with AFP antibody at (A) 1 μg/mL and (B) 2 μg/mL.
Application
Immunofluorescence of AFP in rat liver tissue with AFP antibody at 20 μg/mL.
Application
Immunohistochemistry of AFP in mouse liver tissue with AFP antibody at 2.5 μg/ml.
Application
Immunohistochemistry of AFP in rat liver tissue with AFP antibody at 10 μg/mL.| Application Notes | AFP antibody can be used for detection of AFP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | AFP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AFP Antibody is supplied in PBS containing 0.02% sodium azide. |
