Application
Western blot analysis in K562 cell line lysates (35ug/lane).Application
AGBL5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Confocal immunofluorescent analysis of AGBL5 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | AGBL5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | AGBL5 has a function in the processing of cytosolic proteins such as alpha tubulin, which is known to be modified by the removal of a C terminal tyrosine. It is expressed in the brain. There are three named isoforms.1) Bailey, S.D., et al. Diabetes Care (2010) In press : |
| Immunogen | This AGBL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-127 amino acids from the N-terminal region of human AGBL5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AGBL5 |
|---|---|
| Alternative Names | Cytosolic carboxypeptidase-like protein 5 3417- ATP/GTP-binding protein-like 5 AGBL5 CCP5 |
| Molecular Weight(MW) | 98 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).
Application
AGBL5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Confocal immunofluorescent analysis of AGBL5 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
