Application
Western blot analysis of anti-AGT Antibody in HepG2 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AGT antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | AGT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Angiotensinogen, Serpin A8, Angiotensin-1, Angiotensin 1-10, Angiotensin I, Ang I, Angiotensin-2, Angiotensin 1-8, Angiotensin II, Ang II, Angiotensin-3, Angiotensin 2-8, Angiotensin III, Ang III, Des-Asp[1]-angiotensin II, Angiotensin-4, Angiotensin 3-8, |
| Product description | AGT, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in AGT gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in AGT gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.1) Gurkan,A., Arch. Oral Biol. 54 (4), 337-344 (2009) |
| Immunogen | This AGT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the N-terminal region of human AGT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | AGT |
|---|---|
| Alternative Names | Angiotensinogen Serpin A8 Angiotensin-1 Angiotensin 1-10 Angiotensin I Ang I Angiotensin-2 Angiotensin 1-8 Angiotensin II Ang II Angiotensin-3 Angiotensin 2-8 Angiotensin III Ang III Des-Asp[1]-angiotensin II Angiotensin-4 Angiotensin 3-8 |
| Molecular Weight(MW) | 53 kDa |
Application
Western blot analysis of anti-AGT Antibody in HepG2 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AGT antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
