Application
Immunofluorescence staining of methanol-fixed MCF cells using Progesterone Receptor (Phospho-Ser190) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Progesterone Receptor (Phospho-Ser190) Antibody .Application
Western blot analysis of extracts from SKOV3 cells untreated (lane 1) or treated with EGF (lane 2) using Progesterone Receptor (Phospho-Ser190) Antibody .Application
Western blot analysis of extracts from SKOV3 cells untreated (lane 1) or treated with EGF (lane 2) using Progesterone Receptor (Phospho-Ser190) Antibody| Product Name | Progesterone Receptor (phospho Ser190) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Progesterone Receptor (Phospho Ser190)Progesterone receptors (PRs) are nuclear hormone receptors of the NR3C class, which also includes mineralocorticoid,glucocorticoid and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins, which are shed upon activation. The major signaling pathway used by progesterone receptors is via direct DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins, mainly with transcription factors such as NF-kappaB, AP-1 or STAT. Progesterone receptors are found in the female reproductive tract, mammary glands, brain and pituitary gland and receptor expression is induced by estrogen. Well established functions of progesterone receptors include ovulation, implantation, mammary gland development and maintenance of pregnancy. In addition,progesterone, signaling through the progesterone receptor, increases the ventilatory response of the respiratory centers to carbon dioxide and decreases arterial and alveolar PCO2 in the luteal phase of the menstrual cycle and during pregnancy. The human gene encoding the progesterone receptor has been localized to 11q22. |
| Immunogen | Peptide sequence around phosphorylation site of serine 190 (G-L-S(p)-P-A) derived from Human Progesterone Receptor. |
| Modification | p-Ser190 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NR3C3 PR PGR progesterone receptor nuclear receptor subfamily 3 group C member 3 |
|---|---|
| Target | Progesterone Receptor (Phospho Ser190) |
| Molecular Weight(MW) | 99 (note) |
Application
Immunofluorescence staining of methanol-fixed MCF cells using Progesterone Receptor (Phospho-Ser190) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Progesterone Receptor (Phospho-Ser190) Antibody .
Application
Western blot analysis of extracts from SKOV3 cells untreated (lane 1) or treated with EGF (lane 2) using Progesterone Receptor (Phospho-Ser190) Antibody .
Application
Western blot analysis of extracts from SKOV3 cells untreated (lane 1) or treated with EGF (lane 2) using Progesterone Receptor (Phospho-Ser190) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
