Application
Western blot of T47D cell lysate specific for the human progesterone receptor phosphorylated at Ser294. The antibody specifically reacts with the progesterone receptor phosphorylated at Ser294 in T47D cells.| Product Name | Progesterone Receptor (phospho Ser294) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Progesterone Receptor ( phospho S294 )The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC 1) or corepressor (e.g. NCoR) proteins. |
| Immunogen | Synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer294 of human progesterone receptor |
| Modification | p-Ser294 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotWB |
| Dot: Use at a dilution of 1/1000 - 1/2000. WB: Use at a dilution of 1/1000 - 1/2000. Predicted molecular weight: 112 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | PGR 607311 Progesterone receptor P06401 PR NR3C3 5241 Progesterone receptor (phospho S294) Progesterone receptor (phospho Ser294) Progesterone receptor (pS294) Progesterone receptor (pSer294) Progesterone receptor phospho S294 Progesterone rec |
|---|---|
| Target | Progesterone receptor |
| Tissue Specificity | The antibody is specific for human progesterone receptor phosphorylated at Ser294. |
| Cellular Localization | Nuclear |
Application
Western blot of T47D cell lysate specific for the human progesterone receptor phosphorylated at Ser294. The antibody specifically reacts with the progesterone receptor phosphorylated at Ser294 in T47D cells.| Application Notes | Dot: Use at a dilution of 1/1000 - 1/2000. WB: Use at a dilution of 1/1000 - 1/2000. Predicted molecular weight: 112 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: 50% Glycerol, 150mM Sodium Chloride, 10mM HEPES, 100µg/ml BSA |
