Application
staining Progesterone Receptor in breast carcinoma by Immunohistochemistry (FFPE-sections).Application
Human Breast Carcinoma stained with anti-Progesterone Receptor (PR) antibody| Product Name | Progesterone Receptor antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Progesterone Receptor predilutedThe human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC 1) or corepressor (e.g. NCoR) proteins. |
| Immunogen | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC-P: Working dilution, ready to use for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PGR 607311 Progesterone receptor P06401 PR NR3C3 5241 |
|---|---|
| Target | Progesterone receptor |
| Cellular Localization | Nuclear |
Application
staining Progesterone Receptor in breast carcinoma by Immunohistochemistry (FFPE-sections).
Application
Human Breast Carcinoma stained with anti-Progesterone Receptor (PR) antibody| Positive Control | Breast carcinomas |
|---|---|
| Application Notes | IHC-P: Working dilution, ready to use for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH 7.4 containing 1%BSA and 0.1% sodium azide |
