Application
Western blot analysis of AIFM2 Antibody in mouse liver tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AIFM2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | AIFM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptosis-inducing factor 2, 1---, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis-inducing factor-like mitochondrion-associated inducer of death, p53-responsive gene 3 protein, AIFM2, AMID, PRG3 {ECO:0000303|PubMe |
| Product description | AIFM2 is significant homology to NADH oxidoreductases and the apoptosis-inducing factor PDCD8/AIF. The protein has been shown to induce apoptosis. This protein is found to be induced by tumor suppressor protein p53 in colon caner cells.1) Ohiro Y., Garkavtsev I.FEBS Lett. 524:163-171(2002) |
| Immunogen | This AIFM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-348 amino acids from the C-terminal region of human AIFM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | AIFM2 |
|---|---|
| Alternative Names | Apoptosis-inducing factor 2 1--- Apoptosis-inducing factor homologous mitochondrion-associated inducer of death Apoptosis-inducing factor-like mitochondrion-associated inducer of death p53-responsive gene 3 protein AIFM2 AMID PRG3 {ECO:0000303|PubMe |
| Molecular Weight(MW) | 41 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of AIFM2 Antibody in mouse liver tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AIFM2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
