Application
Western blot analysis of extracts from MCF, PC12, 293 and 3T3 cells using PHB (Phospho-Thr258) Antibody.| Product Name | Prohibitin (phospho Thr258) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Prohibitin (phospho Thr258)This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Immunogen | Peptide sequence around phosphorylation site of threonine 258 (N-I-T(p)-Y-L) derived from Human PHB. |
| Modification | p-Thr258 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PHB1 PHB HEL-S-54e prohibitin HEL-215 |
|---|---|
| Target | Prohibitin (phospho Thr258) |
| Tissue Specificity | The antibody detects endogenous level of PHB only when phosphorylated at threonine 258. |
Application
Western blot analysis of extracts from MCF, PC12, 293 and 3T3 cells using PHB (Phospho-Thr258) Antibody.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C |
| Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
