Application
Western blot analysis of AIMP2 in HeLa cell lysate with AIMP2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of AIMP2 in rat small intestine tissue with AIMP2 antibody at 20 μg/mL.Application
Immunohistochemistry of AIMP2 in rat small intestine tissue with AIMP2 antibody at 5 μg/mL.| Product Name | AIMP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIMP2 Antibody: P38, JTV1, JTV-1, PRO0992, Aminoacyl tRNA synthase complex-interacting multifunctional protein 2, Multisynthase complex auxiliary component p38 |
| Product description | AIMP2 was initially identified as a part of an aminoacyl-tRNA synthesase complex (1). It was later discovered to be a cofactor and substrate of Parkin, a Ring-type E3 ubiquitin ligase that is important for the survival of dopamine neurons in Parkinson’s disease; accumulation of AIMP2 in these cells lead to catecholaminergic cell death (2). AIMP2 can also bind to TRAF2, a key player in the TNF-alpha signaling pathway, causing the ubiquitination of TRAF2 by cIAP1, leading to TNF-alpha-dependent apoptosis (3). Finally, AIMP2 has been suggested to function as a tumor suppressor (4).1) Quevillon S, Robinson JC, Berthonneau E, et al. Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein. J. Mol. Biol. 1999; 285:183-95. |
| Immunogen | AIMP2 antibody was raised against a 16 amino acid peptide near the center of human AIMP2. The immunogen is located within amino acids 150 - 200 of AIMP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AIMP2 antibody can be used for detection of AIMP2 by Western blot at 1 - 2 μg/ml.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AIMP2 |
|---|---|
| Alternative Names | AIMP2 Antibody: P38 JTV1 JTV-1 PRO0992 Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 Multisynthase complex auxiliary component p38 |
| Molecular Weight(MW) | Predicted: 35 kDa Observed: 36 kDa |
| Tissue Specificity | AIMP2 antibody is human specific. AIMP2 antibody is predicted to not cross-react with AIMP1. |
Application
Western blot analysis of AIMP2 in HeLa cell lysate with AIMP2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of AIMP2 in rat small intestine tissue with AIMP2 antibody at 20 μg/mL.
Application
Immunohistochemistry of AIMP2 in rat small intestine tissue with AIMP2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | AIMP2 antibody can be used for detection of AIMP2 by Western blot at 1 - 2 μg/ml.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AIMP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | AIMP2 antibody is supplied in PBS containing 0.02% sodium azide. |
