Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Product Name | AK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AK1, HTL-S-58j |
| Product description | Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Quillen, E.E., (2006) J. Biol. Chem. 281 (30), 20728-20737. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| AK1 antibody can be used for detection of AK1 by ELISA at 1:312500. AK1 antibody can be used for detection of AK1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AK1 |
|---|---|
| Alternative Names | AK1 HTL-S-58j |
| Molecular Weight(MW) | 22 kDa |
Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | AK1 antibody can be used for detection of AK1 by ELISA at 1:312500. AK1 antibody can be used for detection of AK1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store AK1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
