Application
Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: human kidney lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | AK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenylate kinase 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03168}, AK 2 {ECO:0000255|HAMAP-Rule:MF_03168}, 2743 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP-AMP transphosphorylase 2 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP:AMP phosphotransferase {ECO:0000255|HAM |
| Product description | Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis.1) Noma, T., et al., Biochim. Biophys. Acta 1395(1):34-39 (1998). |
| Immunogen | This AK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-217 amino acids from the C-terminal region of human AK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AK2 |
|---|---|
| Alternative Names | Adenylate kinase 2 mitochondrial {ECO:0000255|HAMAP-Rule:MF_03168} AK 2 {ECO:0000255|HAMAP-Rule:MF_03168} 2743 {ECO:0000255|HAMAP-Rule:MF_03168} ATP-AMP transphosphorylase 2 {ECO:0000255|HAMAP-Rule:MF_03168} ATP:AMP phosphotransferase {ECO:0000255|HAM |
| Molecular Weight(MW) | 26 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: human kidney lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
