Application
Antibody is used in Western blot to detect AK3 in mouse kidney tissue lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of AK3 using rabbit polyclonal AK3 Antibody (C-term H38) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AK3 gene (Lane 2).| Product Name | AK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenylate kinase 4, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03170}, AK 4 {ECO:0000255|HAMAP-Rule:MF_03170}, 27410 {ECO:0000255|HAMAP-Rule:MF_03170}, 2746 {ECO:0000255|HAMAP-Rule:MF_03170}, Adenylate kinase 3-like {ECO:0000255|HAMAP-Rule:MF_03170}, GTP:AMP |
| Product description | AK3 is a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated.1) Van Rompay, A.R., et al., Eur. J. Biochem. 261(2):509-517 (1999). |
| Immunogen | This AK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the C-terminal region of human AK3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AK4 |
|---|---|
| Alternative Names | Adenylate kinase 4 mitochondrial {ECO:0000255|HAMAP-Rule:MF_03170} AK 4 {ECO:0000255|HAMAP-Rule:MF_03170} 27410 {ECO:0000255|HAMAP-Rule:MF_03170} 2746 {ECO:0000255|HAMAP-Rule:MF_03170} Adenylate kinase 3-like {ECO:0000255|HAMAP-Rule:MF_03170} GTP:AMP |
| Molecular Weight(MW) | 25 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Antibody is used in Western blot to detect AK3 in mouse kidney tissue lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of AK3 using rabbit polyclonal AK3 Antibody (C-term H38) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AK3 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
