AKAP7 is a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations. Three alternatively spliced transcript variants encoding different isoforms have been described. Additional variants exist, but their full-length natures have not been determined.1) Brown, R.L., et al., (2003) Biochem. Biophys. Res. Commun. 306 (2), 394-401.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AKAP7.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
AKAP7 antibody can be used for detection of AKAP7 by ELISA at 1:312500. AKAP7 antibody can be used for detection of AKAP7 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Application Notes
AKAP7 antibody can be used for detection of AKAP7 by ELISA at 1:312500. AKAP7 antibody can be used for detection of AKAP7 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store AKAP7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.