Application
Confocal immunofluorescent analysis of AKR1B1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-AKR1B1 Pab in Jurkat cell line lysates (35ug/lane)Application
Western blot analysis of AKR1B1 using rabbit polyclonal AKR1B1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.Application
Western Blot at 1:8000 dilution Lane 1: A431 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | AKR1B1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aldose reductase, AR, Aldehyde reductase, Aldo-keto reductase family 1 member B1, AKR1B1, ALDR1 |
| Product description | AKR1B1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This protein catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol.1) Steuber,H.,J. Mol. Biol. 379 (5), 991-1016 (2008) |
| Immunogen | This AKR1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-135 amino acids from the Central region of human AKR1B1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | AKR1B1 |
|---|---|
| Alternative Names | Aldose reductase AR Aldehyde reductase Aldo-keto reductase family 1 member B1 AKR1B1 ALDR1 |
| Molecular Weight(MW) | 36 kDa |
Application
Confocal immunofluorescent analysis of AKR1B1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AKR1B1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-AKR1B1 Pab in Jurkat cell line lysates (35ug/lane)
Application
Western blot analysis of AKR1B1 using rabbit polyclonal AKR1B1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1B1 gene.
Application
Western Blot at 1:8000 dilution Lane 1: A431 whole cell lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
