Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).Application
AKR1C2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | AKR1C2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aldo-keto reductase family 1 member C2, 1---, 3-alpha-HSD3, Chlordecone reductase homolog HAKRD, Dihydrodiol dehydrogenase 2, DD-2, DD2, Dihydrodiol dehydrogenase/bile acid-binding protein, DD/BABP, Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, Type III |
| Product description | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.1) Setlur, S.R., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):229-239(2010) |
| Immunogen | This AKR1C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-323 amino acids from the C-terminal region of human AKR1C2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKR1C2 |
|---|---|
| Alternative Names | Aldo-keto reductase family 1 member C2 1--- 3-alpha-HSD3 Chlordecone reductase homolog HAKRD Dihydrodiol dehydrogenase 2 DD-2 DD2 Dihydrodiol dehydrogenase/bile acid-binding protein DD/BABP Trans-1 2-dihydrobenzene-1 2-diol dehydrogenase Type III |
| Molecular Weight(MW) | 37 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application
AKR1C2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
