Application
Western blot analysis of extracts from A549 cells treated with PMA using Phospho-Akt (Ser129) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Akt (phospho Ser129) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT, AKT1 kinase, kinase Akt1, RAC, RAC-PK-alpha |
| Product description | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.1) Nguyen le XT, Mitchell BS (2013) Proc Natl Acad Sci U S A 110, 20681-6 |
| Immunogen | Akt (phospho Ser129) antibody was raised against a peptide sequence around phosphorylation site of serine129 (D-N-S (p)-G-A) derived from Human Akt . |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 |
|---|---|
| Alternative Names | AKT AKT1 kinase kinase Akt1 RAC RAC-PK-alpha |
| Molecular Weight(MW) | 65 kDa |
| Tissue Specificity | The antibody detects endogenous level of Akt only when phosphorylated at serine 129. |
| Entrez Gene | 207 |
|---|---|
| Protein Accession | NP_001014431.1 |
Application
Western blot analysis of extracts from A549 cells treated with PMA using Phospho-Akt (Ser129) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM270697