Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF, Serum or both using Akt (Phospho-Ser473).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser473).WB
(C–E) The phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473) and AKT (phospho Thr308) was markedly reduced by HG inducement.WB
(D, E) DOP treatment markedly relived HG-induced suppressive effects on the phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473), AKT (phospho Thr308) and Nephrin.WB
(C, D) IRS-1/2 inhibitor NT157 treatment notably reversed HG-induced increased phosphorylation of IRS-1 (phospho Y632), AKT (phospho Ser473), AKT (phospho Thr308) and Nephrin.| Product Name | Akt (phospho Ser473) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.1) Baudhuin LM, et al. (2004) FASEB J Feb; 18 (2): 341-3. Min YH, et al. (2004) Cancer Res; 64 (15): 5225-31. Feng J, et al. (2004) J Biol Chem; 279 (34): 35510-7. |
| Immunogen | Akt (Phospho-Ser473) antibody was raised against a peptide sequence around phosphorylation site of serine 473 (Q-F-S (p) -Y-S) derived from Human Akt. |
| Modification | p-Ser473 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 |
|---|---|
| Alternative Names | AKT PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA NFKB3 C-AKT PKB-alpha RAC-PK-alpha |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of Akt only when phosphorylated at serine 473. |
| Entrez Gene | 207 |
|---|---|
| Protein Accession | NP_001014431.1 |
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF, Serum or both using Akt (Phospho-Ser473).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser473).
WB
(C–E) The phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473) and AKT (phospho Thr308) was markedly reduced by HG inducement.
WB
(D, E) DOP treatment markedly relived HG-induced suppressive effects on the phosphorylation of insulin receptor substrate (IRS)-1 (phospho Y632), AKT (phospho Ser473), AKT (phospho Thr308) and Nephrin.
WB
(C, D) IRS-1/2 inhibitor NT157 treatment notably reversed HG-induced increased phosphorylation of IRS-1 (phospho Y632), AKT (phospho Ser473), AKT (phospho Thr308) and Nephrin.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
