Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear dot staining using Akt (Phospho-Thr308).| Product Name | Akt (phospho Thr308) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, CFL, C-AKT, PKB-alpha, RAC-PK-alpha |
| Product description | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.1) Tremblay F, et al. (2005) Diabetes; 54 (9): 2674-84. Xu BE, et al. (2005) J Biol Chem; 280 (40): 34218-23. Samuels Y, et al. (2005) Cancer Cell; 7 (6): 561-73. Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77. |
| Immunogen | Akt (Phospho-Thr308) antibody was raised against a peptide sequence around phosphorylation site of threonine 308 (M-K-T (p) -F-C) derived from Human Akt. |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 |
|---|---|
| Alternative Names | AKT PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA CFL C-AKT PKB-alpha RAC-PK-alpha |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of Akt only when phosphorylated at threonine 308. |
| Entrez Gene | 207 |
|---|---|
| Protein Accession | NP_001014431.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear dot staining using Akt (Phospho-Thr308).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM270700