Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using AKT1 (phospho-Thr450).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (Phospho-Thr450). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AKT1 (phospho-Thr450).| Product Name | AKT1 (phospho Thr450) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, CTNNB, OK/SW-cl.35, PRO2286, RAC-PK-alpha, Protein kinase B |
| Product description | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.1) Xing J, et al. (1998) Mol Cell Biol 18 (4): 1946-55. Tan Y, et al. (1996) EMBO J; 15 (17): 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276 (27): 25184-9. |
| Immunogen | AKT1 (phospho-Thr450) antibody was raised against a peptide sequence around phosphorylation site of threonine 450 (T-I-T (p) -P-P) derived from Human AKT1. |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 |
|---|---|
| Alternative Names | AKT PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA CTNNB OK/SW-cl.35 PRO2286 RAC-PK-alpha Protein kinase B |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of AKT1 only when phosphorylated at threonine 450. |
| Entrez Gene | 207 |
|---|---|
| Protein Accession | NP_001014431.1 |
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using AKT1 (phospho-Thr450).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (Phospho-Thr450). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AKT1 (phospho-Thr450).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM270707