AKT1 is a general protein kinase capable of phosphorylating several known proteins.1) Jones, P.F., et al., (1991) Proc. Natl. Acad. Sci. U.S.A. 88: 4171-4175..
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AKT1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
AKT1 antibody can be used for detection of AKT1 by ELISA at 1:62500. AKT1 antibody can be used for detection of AKT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Small Intestine at 0.2-1 ug/ml.
Application Notes
AKT1 antibody can be used for detection of AKT1 by ELISA at 1:62500. AKT1 antibody can be used for detection of AKT1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store AKT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt