Application
Immunohistochemistry staining of AKT1 in cerebellum tissue using AKT1 Antibody.| Product Name | AKT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA |
| Product description | AKT1 encodes a RAC/AKT-type protein kinase that contains a N-terminal pleckstrin-homology (PH) domain and a central catalytic domain closely related to both cAMP-dependent protein kinase and protein kinase C. The protein is a member of PI3K-mediated signaling pathways associated with the regulation of proliferation, survival, protein synthesis, and metabolism. It is phosphorylated by 3'-phosphoinositide-dependent kinase-1 (PDK1) in response to stimulation by growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and insulin. Activated AKT1 has been shown to phosphorylate and inactivate components of the apoptotic machinery. AKT1 (-/-) mice have been shown to be viable but smaller than wild-type littermates, and they have a reduced lifespan and increased spontaneous apoptosis in several tissues when exposed to stressors. AKT1 protein is the cellular homolog of the product of the retroviral oncogene v-akt, and has been implicated in numerous cancers. |
| Immunogen | AKT1 antibody was raised against amino acids surrounding threonine 308 of AKT1 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| AKT1 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, immunofluorescence starting at 10 μg/mL, and immunoprecipitation.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 |
|---|---|
| Alternative Names | AKT1 AKT AKT-1 C-AKT Pan-AKT PKB alpha PKBalpha Proto-oncogene c-Akt Protein kinase B Rac protein kinase alpha PRKBA Protein kinase B alpha RAC-PK-alpha PKB PKB-ALPHA RAC RAC-ALPHA |
| Tissue Specificity | pThr308 |
Application
Immunohistochemistry staining of AKT1 in cerebellum tissue using AKT1 Antibody.| Application Notes | AKT1 antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, immunofluorescence starting at 10 μg/mL, and immunoprecipitation.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | AKT1 antibody should be stored long term (months) at -80 ˚C and short term (days) at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.02% sodium azide, 50% glycerol. |
Data sheet for OM270717