Application
Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE diluted at 1:5000| Product Name | Proteasome 20S beta 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Proteasome 20S beta 3 (proteasome (prosome, macropain) subunit, beta type, 3)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Pseudogenes have been identified on chromosomes 2 and 12. [provided by RefSeq] |
| Immunogen | Full length human Proteasome 20S beta 3 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Dog, Pig, Rhesus Monkey, BovineMouse (98%), Rat (98%), Zebrafish (90%), Xenopus laevis (91%), Dog (99%), Pig (97%), Rhesus Monkey (99%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HC10-II antibody MGC4147 antibody PSMB3 antibody proteasome chain 13 antibody proteasome component C10-II antibody proteasome subunit beta type-3 antibody proteasome theta chain antibody "proteasome (prosome macropain) subunit beta type 3 antibod |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE diluted at 1:5000| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
