Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000| Product Name | Proteasome beta 4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Proteasome beta 4 (proteasome (prosome, macropain) subunit, beta type, 4)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Proteasome beta 4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rabbit, Rhesus Monkey, BovineMouse (96%), Rat (94%), Pig (96%), Rabbit (96%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HN3 antibody HsN3 antibody PROS-26 antibody PROS26 antibody PSMB4 antibody macropain beta chain antibody "proteasome subunit beta type 4 antibody" multicatalytic endopeptidase complex beta chain antibody proteasome beta chain antibody 26 kDa pro |
|---|---|
| Target | Proteasome beta 4 |
| Molecular Weight(MW) | 29 kDa (note) |
| Cellular Localization | Cytoplasm (By similarity) , Nucleus |
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000| Positive Control | A431 , H1299 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
