Application
Western blot analysis of lysates from A549 cell line and rat liver tissue lysate (from left to right), using ALDH1A1 Antibody at 1:1000 at each lane.Application
Fluorescent image of A549 cells stained with ALDH1A1 Antibody . AP20580c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Product Name | ALDH1A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Retinal dehydrogenase 1, RALDH 1, RalDH1, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic, ALDH1A1, ALDC, ALDH1, PUMB1 |
| Product description | Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity).1) Hsu L.C.,et al.Genomics 5:857-865(1989). |
| Immunogen | This ALDH1A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 306-339 amino acids from the Central region of human ALDH1A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For IF starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALDH1A1 |
|---|---|
| Alternative Names | Retinal dehydrogenase 1 RALDH 1 RalDH1 ALDH-E1 ALHDII Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase cytosolic ALDH1A1 ALDC ALDH1 PUMB1 |
| Molecular Weight(MW) | 55 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis of lysates from A549 cell line and rat liver tissue lysate (from left to right), using ALDH1A1 Antibody at 1:1000 at each lane.
Application
Fluorescent image of A549 cells stained with ALDH1A1 Antibody . AP20580c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Application Notes | For IF starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
