The aldehyde dehydrogenases are a family of isozymes that may play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. ALDH3B1 is highly expressed in kidney and lung. The aldehyde dehydrogenases are a family of isozymes that may play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular gene spans about 20 kb of genomic DNA and is composed of 9 coding exons. The gene encodes a single transcript of 2.8 kb which is highly expressed in kidney and lung. The functional significance of this gene as well as the cellular localization of its product are presently unknown. Two transcript variants encoding different isoforms have been found for this gene.1) Marchitti, S.A., (2007) Biochem. Biophys. Res. Commun. 356 (3), 792-798.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALDH3B1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ALDH3B1 antibody can be used for detection of ALDH3B1 by ELISA at 1:62500. ALDH3B1 antibody can be used for detection of ALDH3B1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on Human Jurkat 1 ug/ml.
Application
Antibody used in WB on Human HeLa cells at 1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
ALDH3B1 antibody can be used for detection of ALDH3B1 by ELISA at 1:62500. ALDH3B1 antibody can be used for detection of ALDH3B1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ALDH3B1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.