Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | ALF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALF, ALF |
| Product description | The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. ALF is a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.1) Upadhyaya, A.B., et al., (2002) J. Biol. Chem. 277 (37), 34208-34216. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ALF antibody can be used for detection of ALF by ELISA at 1:312500. ALF antibody can be used for detection of ALF by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GTF2A1L |
|---|---|
| Alternative Names | ALF ALF |
| Molecular Weight(MW) | 50 kDa, 52 kDa, 132 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ALF antibody can be used for detection of ALF by ELISA at 1:312500. ALF antibody can be used for detection of ALF by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ALF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
