Application
Western blot analysis of ALG10 Antibody in A375 cell line lysates (35ug/lane)Application
ALG10 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ALG10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase, Alpha-1,2-glucosyltransferase ALG10-A, Alpha-2-glucosyltransferase ALG10-A, Asparagine-linked glycosylation protein 10 homolog A, ALG10, ALG10A |
| Product description | ALG10 encodes a membrane-associated protein that adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. That is, it transfers the terminal glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc2Man9GlcNAc(2)-PP-Dol. The rat protein homolog was shown to specifically modulate the gating function of the rat neuronal ether-a-go-go (EAG) potassium ion channel.1) Chapuis, J., et al. Mol. Psychiatry 14(11):1004-1016(2009) |
| Immunogen | This ALG10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-43 amino acids from the N-terminal region of human ALG10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALG10 |
|---|---|
| Alternative Names | Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1 2-glucosyltransferase Alpha-1 2-glucosyltransferase ALG10-A Alpha-2-glucosyltransferase ALG10-A Asparagine-linked glycosylation protein 10 homolog A ALG10 ALG10A |
| Molecular Weight(MW) | 56 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of ALG10 Antibody in A375 cell line lysates (35ug/lane)
Application
ALG10 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
