Application
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ALKBH8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alkylated DNA repair protein alkB homolog 8, 11411-, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8, ALKBH8, ABH8 |
| Product description | ALKBH8 may inhibit apoptosis and promote cell survival and angiogenesis.1) Shimada,K., et.al., Cancer Res. 69 (7), 3157-3164 (2009) |
| Immunogen | This ALKBH8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from the C-terminal region of human ALKBH8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ALKBH8 |
|---|---|
| Alternative Names | Alkylated DNA repair protein alkB homolog 8 11411- Probable alpha-ketoglutarate-dependent dioxygenase ABH8 S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8 tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8 ALKBH8 ABH8 |
| Molecular Weight(MW) | 75 kDa |
Application
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
