Application
Western blot analysis of alpha-tubulin in human brain tissue lysate with alpha-tubulin antibody at (A) 0.25 and (B) 0.5 μg/ml.Application
Immunohistochemistry of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 5 μg/mL.Application
Immunofluorescence of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 20 μg/mL.| Product Name | Alpha-tubulin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2 |
| Product description | Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.1) McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33. |
| Immunogen | Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin. The immunogen is located within the first 50 amino acids of Alpha-tubulin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TUBA3C |
|---|---|
| Alternative Names | Alpha-tubulin Antibody: TUBA2 bA408E5.3 TUBA2 TUBA3D Alpha-tubulin 2 |
| Molecular Weight(MW) | Predicted: 50 kDa Observed: 45 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | Alpha-tubulin antibody is human, mouse and rat reactive. |
Application
Western blot analysis of alpha-tubulin in human brain tissue lysate with alpha-tubulin antibody at (A) 0.25 and (B) 0.5 μg/ml.
Application
Immunohistochemistry of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 5 μg/mL.
Application
Immunofluorescence of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Alpha-tubulin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide. |
