Application
Western blot analysis of AMY1A Antibody in HL-60 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with AMY1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | AMY1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-amylase 1,4-alpha-D-glucan glucanohydrolase 1, Salivary alpha-amylase, AMY1A, AMY1 |
| Product description | Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. It is an amylase isoenzyme produced by the salivary gland.1) Ragunath, C., et al. J. Mol. Biol. 384(5):1232-1248(2008) |
| Immunogen | This AMY1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-223 amino acids from the Central region of human AMY1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AMY1A |
|---|---|
| Alternative Names | Alpha-amylase 1 4-alpha-D-glucan glucanohydrolase 1 Salivary alpha-amylase AMY1A AMY1 |
| Molecular Weight(MW) | 58 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig |
Application
Western blot analysis of AMY1A Antibody in HL-60 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with AMY1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
