Application
Western blot analysis of ANGPT2 in human liver tissue lysate with ANGPT2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of ANGPT2 in mouse liver tissue with ANGPT2 antibody at 2.5 μg/mL.| Product Name | ANGPT2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANGPT2 Antibody: ANG2, AGPT2, Angiopoietin-2, ANG-2 |
| Product description | ANGPT2 Antibody: Angiopoietin-2 (ANGPT2) is a member of the Ang family, a family of angiogenic factors that play major roles in angiogenesis during the development and growth of human cancers, but also during lymphangiogenesis. ANGPT2 is generally considered an antagonist of ANGPT1 and endothelial TEK tyrosine kinase (TIE-2, TEK). ANGPT2 disrupts the vascular remodeling ability of ANGPT1 and is thought to induce endothelial cell apoptosis, resulting in vessel regression. Expression of ANGPT2 has been linked to invasive and metastatic phenotypes of gliomas and other cancers.1) Hu B and Cheng SY. Angiopoetin-2: Development of inhibitors for cancer therapy. Curr. Oncol. Rep. 2009; 11:111-6. |
| Immunogen | ANGPT2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ANGPT2. The immunogen is located within the last 50 amino acids of ANGPT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ANGPT2 antibody can be used for detection of ANGPT2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANGPT2 |
|---|---|
| Alternative Names | ANGPT2 Antibody: ANG2 AGPT2 Angiopoietin-2 ANG-2 |
| Molecular Weight(MW) | Predicted: 55 kDa Observed: 58 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | At least three isoforms of ANGPT2 are known to exist; this antibody will detect all three isoforms. |
Application
Western blot analysis of ANGPT2 in human liver tissue lysate with ANGPT2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of ANGPT2 in mouse liver tissue with ANGPT2 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | ANGPT2 antibody can be used for detection of ANGPT2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ANGPT2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ANGPT2 Antibody is supplied in PBS containing 0.02% sodium azide. |
