Application
Western blot analysis of ANGPTL3 in human heart tissue lysate with ANGPTL3 antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of ANGPTL3 in rat heart tissue with ANGPTL3 antibody at 20 μg/mL.Application
Immunohistochemistry of ANGPTL3 in rat heart tissue with ANGPTL3 antibody at 5 μg/mL.| Product Name | ANGPTL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANGPTL3 Antibody: ANL3, ANG-5, FHBL2, ANGPT5, UNQ153/PRO179, Angiopoietin-related protein 3, Angiopoietin-5 |
| Product description | The angiopoietin-related protein 3 (ANGPTL3) is a member of a family of secreted proteins that function in angiogenesis (1). ANGPTL3 is processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metablism, while the C-terminal chain may be involved in angiogenesis (2,3). Recent experiments have shown that a deficiency of ANGPTL3 is associated with increased insulin sensitivity, lipoprotein lipase activity, and decreased serum free fatty acids, suggesting that ANGPTL3 may also play a role in modulating glucose metabolism (4).1) Conklin D, Gilbertson D, Taft DW, et al. Identification of a mammalian angiopoietin-related protein expressed specifically in liver. Genomics 1999; 62:477-82. |
| Immunogen | ANGPTL3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ANGPTL3. The immunogen is located within the last 50 amino acids of ANGPTL3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ANGPTL3 antibody can be used for detection of ANGPTL3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANGPTL3 |
|---|---|
| Alternative Names | ANGPTL3 Antibody: ANL3 ANG-5 FHBL2 ANGPT5 UNQ153/PRO179 Angiopoietin-related protein 3 Angiopoietin-5 |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 53 kDa |
| Tissue Specificity | ANGPTL3 antibody is human, mouse and rat reactive. ANGPTL3 antibody is predicted not to cross-react with other ANGPTL family proteins. |
Application
Western blot analysis of ANGPTL3 in human heart tissue lysate with ANGPTL3 antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of ANGPTL3 in rat heart tissue with ANGPTL3 antibody at 20 μg/mL.
Application
Immunohistochemistry of ANGPTL3 in rat heart tissue with ANGPTL3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | ANGPTL3 antibody can be used for detection of ANGPTL3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ANGPTL3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ANGPTL3 antibody is supplied in PBS containing 0.02% sodium azide. |
