Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ANGPTL4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgApplication
Western Blot at 1:1000 dilution Lane 1: human skeletal muscle lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.| Product Name | ANGPTL4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein, HFARP, ANGPTL4, ARP4, HFARP, PGAR |
| Product description | ANGPTL4 is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This protein is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. The encoded protein is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells. The encoded protein may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes.1) Maxwell,T.J., et.al., IntMol Sci 11 (1), 370-385 (2010) |
| Immunogen | This ANGPTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the Central region of human ANGPTL4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANGPTL4 |
|---|---|
| Alternative Names | Angiopoietin-related protein 4 Angiopoietin-like protein 4 Hepatic fibrinogen/angiopoietin-related protein HFARP ANGPTL4 ARP4 HFARP PGAR |
| Molecular Weight(MW) | 45 kDa |
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ANGPTL4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Overlay histogram showing A549 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig
Application
Western Blot at 1:1000 dilution Lane 1: human skeletal muscle lysate Lane 2: human liver lysate Lysates/proteins at 20 ug per lane.| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
