Application
Antibody used in WB on Mouse cortex brain at: 1:1000 (Lane1: 10ug mouse cortex brain lysate, Lane2: 25ug mouse cortex brain lysate, Lane3: 40ug mouse cortex brain lysate).Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in IHC on human kidney at 4.0-8.0 ug/ml.| Product Name | ANP32A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANP32A, LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1 |
| Product description | ANP32A is a novel potent heat-stable inhibitor protein of protein phosphatase 2A. It may play a key role in self-renewing cell populations where it may act in the nucleus to limit their sensitivity to transformation. Being a tumor suppressor, ANP32A represses cell growth through inhibition of transcription by blocking acetylation and phosphorylation of histone H3 and initiating its proapoptotic activity.1) Stelzl, U., (2005) Cell 122 (6), 957-968. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANP32A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| ANP32A antibody can be used for detection of ANP32A by ELISA at 1:312500. ANP32A antibody can be used for detection of ANP32A by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANP32A |
|---|---|
| Alternative Names | ANP32A LANP MAPM PP32 HPPCn PHAP1 PHAPI I1PP2A C15orf1 |
| Molecular Weight(MW) | 29 kDa |
Application
Antibody used in WB on Mouse cortex brain at: 1:1000 (Lane1: 10ug mouse cortex brain lysate, Lane2: 25ug mouse cortex brain lysate, Lane3: 40ug mouse cortex brain lysate).
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in IHC on human kidney at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ANP32A antibody can be used for detection of ANP32A by ELISA at 1:312500. ANP32A antibody can be used for detection of ANP32A by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANP32A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
