Annexin A4 (ANXA4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANXA4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANXA4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANXA4 is almost exclusively expressed in epithelial cells.1) Zimmermann, U., et al., (2004) Cancer Lett. 209 (1), 111-118.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA4.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
ANXA4 antibody can be used for detection of ANXA4 by ELISA at 1:1562500. ANXA4 antibody can be used for detection of ANXA4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Placenta at 1.25 ug/ml.
Positive Control
1) Cat. No. 1309 - Human Placenta Lysate
Application Notes
ANXA4 antibody can be used for detection of ANXA4 by ELISA at 1:1562500. ANXA4 antibody can be used for detection of ANXA4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANXA4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt