ANXA5 belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.1) Kuijpers, T.W., et al., (2004) Blood 103 (10), 3915-3923.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
ANXA5 antibody can be used for detection of ANXA5 by ELISA at 1:1562500. ANXA5 antibody can be used for detection of ANXA5 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Placenta at 0.625 ug/ml.
Positive Control
1) Cat. No. 1309 - Human Placenta Lysate
Application Notes
ANXA5 antibody can be used for detection of ANXA5 by ELISA at 1:1562500. ANXA5 antibody can be used for detection of ANXA5 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANXA5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt