Rabbit Polyclonal antibody to PRP18Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008]
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRPF18. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IPWB
IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMouseMonkeyRatBovine
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
PRPF18 pre-mRNA processing factor 18 hPrp18
Target
PRP18
Tissue Specificity
Recognizes endogenous levels of PRPF18 protein.
Application
Application
Western blot analysis of PRPF18 expression in Jurkat (A); MCF7 (B) whole cell lysates.
Application Notes
IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.