Application
Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE diluted at 1:500| Product Name | PRP8 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PRP8 (PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae))Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PRP8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HPRP8 antibody PRP8 antibody PRPC8 antibody RP13 antibody PRPF8 antibody splicing factor Prp8 antibody "U5 snRNP-specific protein (220 kD) ortholog of S. cerevisiae Prp8p antibody" PRP8 homolog antibody p220 antibody precursor mRNA processing pro |
|---|---|
| Target | PRP8 |
| Molecular Weight(MW) | 274 kDa (note) |
| Cellular Localization | Nucleus speckle |
Application
Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE diluted at 1:500| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
