Application
Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of APC1 in human brain tissue with APC1 antibody at 5 μg/mL.| Product Name | APC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC1 Antibody: APC1, MCPR, TSG24, Anaphase-promoting complex subunit 1, Cyclosome subunit 1, APC1 |
| Product description | APC1 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC1, also known as mitotic checkpoint regulator (MCPR), is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex.1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. |
| Immunogen | APC1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human APC1. The immunogen is located within amino acids 1810 - 1860 of APC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| APC1 antibody can be used for detection of APC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANAPC1 |
|---|---|
| Alternative Names | APC1 Antibody: APC1 MCPR TSG24 Anaphase-promoting complex subunit 1 Cyclosome subunit 1 APC1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of APC1 in SK-N-SH cell lysate with APC1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of APC1 in human brain tissue with APC1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | APC1 antibody can be used for detection of APC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
