Application
Western blot analysis of APC6 in human liver tissue lysate with APC6 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of APC6 in human liver tissue with APC6 antibody at 20 μg/mL.Application
Immunohistochemistry of APC6 in human liver tissue with APC6 antibody at 5 μg/mL.| Product Name | APC6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC6 Antibody: APC6, CUT9, ANAPC6, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 6, APC6 |
| Product description | APC6 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC6 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC6, it has been shown to interact with and is stabilized by CDC26 through an intermolecular TPR mimic composed of one helix from each protein.1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. |
| Immunogen | APC6 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human APC6. The immunogen is located within the first 50 amino acids of APC6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| APC6 antibody can be used for detection of APC6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC16 |
|---|---|
| Alternative Names | APC6 Antibody: APC6 CUT9 ANAPC6 Cell division cycle protein 16 homolog Anaphase-promoting complex subunit 6 APC6 |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Western blot analysis of APC6 in human liver tissue lysate with APC6 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of APC6 in human liver tissue with APC6 antibody at 20 μg/mL.
Application
Immunohistochemistry of APC6 in human liver tissue with APC6 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | APC6 antibody can be used for detection of APC6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APC6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APC6 Antibody is supplied in PBS containing 0.02% sodium azide. |
