Application
Immunocytochemistry of APC8 in K562 cells with APC8 antibody at 5 μg/mL.Application
Western blot analysis of APC8 in K562 cell lysate with APC8 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | APC8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC8 Antibody: APC8, CUT23, ANAPC8, Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, APC8 |
| Product description | APC8 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC8 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. |
| Immunogen | APC8 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC8. The immunogen is located within amino acids 270 - 320 of APC8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| APC8 antibody can be used for detection of APC8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC23 |
|---|---|
| Alternative Names | APC8 Antibody: APC8 CUT23 ANAPC8 Cell division cycle protein 23 homolog Anaphase-promoting complex subunit 8 APC8 |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunocytochemistry of APC8 in K562 cells with APC8 antibody at 5 μg/mL.
Application
Western blot analysis of APC8 in K562 cell lysate with APC8 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | APC8 antibody can be used for detection of APC8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APC8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APC8 Antibody is supplied in PBS containing 0.02% sodium azide. |
